Description
• These cell lines stably express one or more reporter molecules.
The 293 cell line is a permanent line established from primary embryonic human kidney transformed with human adenovirus type 5 DNA. The genes encoded by the E1 region of adenovirus (E1a and E1b) are expressed in these cells and participate in transactivation of viral promoters, allowing these cells to produce high levels of protein. E1 also complements the E1-deletion in recombinant adenoviral vectors, allowing viral replication. Our 293/GFP cell line stably expresses GFP and blasticidin-resistant genes. Both GFP and blasticidin-resistant genes are introduced into parental 293 cells using lentivirus